This project will address the issue of Antibody-mediated rejection (ABMR) after kidney transplantation (KTX) as the major issue of organ rejection. Therefore the project team will develop an automated cell culture device, a corresponding assay to test for the occurrence of ABMR in vitro bevor KTX and implement a data pipeline to integrate the assay data into Laboratory Information and Management System (LIMS) and Clinical Information System (CIS). Therefore, a microfluidic device, and protocols to cultivate specific cells of donor and recipient within that device should be developed in the project. Furthermore, cellular response will be tracked by several automated readout methods. The readout devices will be implemented into the (LIMS) to directly connect data of the assay with the involved clinicians via CIS. Thus it not only provides extra information to existing tests, like HLA crossmatching, but additionally helps the doctor to quickly estimate the probability of ABMR on the basis of a laboratory test, virtually bedside.